Today I will be writing about a Chromatography lab, how it was performed, the results and the analysis. This Chromatography lab was done to identify pigments in different colored leaves and to evaluate the relationship between the pigments and photosynthesis. Chromatography allows a sample, the solute, to be separated out into the samples different pigments and colors through the use of a solvent and capillary action.
Chromatography Lab Answers Purpose The purpose of the experiment is to determine the specific types of pigments found in a beet leaf and in a spinach leaf by using paper chromatography and two solvents: Hypothesis If a water soluble solvent is present, then there will be the movement of only the water soluble pigments up the chromatography paper.
Through capillary action these pigments will travel up the paper until the bonds between the water and pigment become so weak that the pigment must break the attraction and leave itself imprinted at a certain height up the paper.
Therefore, they will remain at the concentrated area unless a lipid soluble solvent is present. If this lipid soluble solvent is present, as opposed to the water soluble solvent, then the lipid soluble pigments will move up the chromatography paper instead of the water soluble pigments.
The same idea will happen. The lipid soluble pigments will travel up the paper until their bonds between the water are so Bio2133 lab chromatogram lab report that it must stop following the movement of the solvent, and get placed at a certain height above the original concentrated dot.
Spinach will have mainly chlorophyll A and B because the leaf is completely green, compared to the beet leaf which consists of both a red and green shade showing that other pigments are present in this leaf.
Chlorophyll a and b are two common types of chlorophyll found on the thylakoid membrane in several photosynthetic units. Both have a similar purpose: The molecules do this by absorbing specific wavelenghts of light rays red and blue-violet light which excite electrons contained within the double bonds of the porphyrin ring of the chlorophyll molecule.
Chlorophyll b passes its exited electrons to chlorophyll a, which passes its excited electrons to other molecules, which store the energy as chemical potential energy. This is the base step of photosynthesis. Cholorphyll b is an accessory pigmant, meaning it always passes its excited electrons to chlorophyll a.
Chlorophyll a is the main absorber, meaning only it can pass the excited electrons to other molecules. Because leaves are very rich in chloroplasts containing chlorophyll for photosynthesis photosynthesis occurs mainly on leavesthey reflect a lot of green light wavelengths, which our eyes interpret as the colour green.
As the fall comes so do the shorter days. This results in less light during the day for the plant to undergo photosynthesis. This allows the accessory pigments carotene, xanthophyll to take over the light capturing process because they are able to trap different wavelengths of light.
The combined trapping ability of the accessory pigments and the regular pigments chlorophyll A and B allows for a greater combined absorption of light, because there are more pigments trapping a greater range of light from the visible light spectrum.
This results in the plant being able to perform more photosynthesis because more photons of light are being absorbed which will result in more glucose being formed which will ultimately be stored as potential energy in the form of sugars during the winter months when photosynthesis doesnt occur.
Paper chromatography is a process in which pigments are separated, from an initial concentrated solution, through the process of capillary action. A solvent is placed at the bottom of the paper. As the solution travels up the paper, like soluble pigments will travel with the solvent until the bonds between the solvent and pigment become so weak that it must break the attraction and imprint itself a certain height up the paper.
Some factors that affect paper chromatography are: A certain solvent will only attract certain pigments up the paper. For example this experiment used water soluble and lipid soluble solvents.
This means that when one of these solvents are present in the trial, only that type of pigment will travel with the movement of the solvent lipid soluble solvent with lipid soluble pigment and water soluble solvent with water soluble pigment.
The beet leaf contained more pigments in the leaf compared to the spinach leaf. This could be because the beet has a root where it stores starch.
This would require it to undergo photosynthesis more times in order for its to create more glucose to store. The increased number of accessory pigments allows for a wider range of light to be taken in and converted into energy excitation of electrons on the chlorophyll for the photosynthetic process.
Conclusion The experiment was carried out and it was proven that: Chlorophyll A and B.
This was shown when the paper chromatography was done, the beet leaf trials had many different coloured pigments over the paper, vs the spinach leaf only a green pigment which represented that only chlorophyll was present.Here is the best resource for homework help with BIO GENETICS at University Of Ottawa.
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Banana Oil Lab Report Jesse Bradford 7/10/14 MTWR Section Introduction In the banana oil lab we began with isopentyl alcohol + acetic acid isopentyl acetate + Water.
We needed for this experiment a hot plate, clamps, pipette, 5mL vial, caps, hoses and a thermometer. View Notes - BIO LAB - CHROMATOGRAM LAB REPORT from BIO at University of Ottawa.
Figure 1 compares the presence of various eye pigments found in . Materials The materials used for this lab are paper, pencil, eraser, filter paper, test tube, rubber stopper, paper clip, metric ruler, black felt-tip pen, and a computer.
Paper Chromatography Table of Contents. Introduction.
Experimental. BIOTECHNOLOGY LAB REPORT FORMAT GENERAL INFORMATION: ALL lab reports follow the same classical organization and format. They should be neatly written in your science lab notebook. Every section of the lab report should be written in.